How much caffeine in coffee?

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[ Applause ] I used to drink a lot of coffee and my hand shook even more than they do now about 12 years ago. Maybe more now I went to China for a week and when I left I was really addicted to coffee and when I came back I was found myself addicted to green tea. And since then, I drank nearly entirely green tea and very rarely do I drink coffee, and the result is that if I do have some coffee, I feel hyper for the rest of the day and I really can’t sleep well, it’s a molecule.

I know – and I can just about recognize its structure together – some carbons, carbonless we’ve got nitrogen, we’ve got oxygen and hydrogen scattering atoms everywhere. If I just saw it as a random molecule, I would probably recognize it because you usually see it on people’s coffee cups and things like that.

So there’s quite a good thing. This is cupping. If you’ve never had coffee before, then you probably do get some stimulation from it. There’S no doubt about that’s been proven. It can help making a bit more alert and feel a bit bit more vital for a few minutes. If you have too much, of course, a lethal dose of coffee is about 80 cups a day, so do not approach that at all. I have read the Tyrian.

I don’t know if this is correct, that if you take coffee or and some other caffeinated drink at a regular time each day, then your body starts making the antidote to the caffeine before you get to coffee time. So if you have coffee at 10:30 in the morning round about 10 o’clock, your body starts making this antidote, so you’ve tend to feel really quite in need of the caffeine just to get you back to normal state. So this is about six cups of coffee.

In here and we’re just going to cool that down, but on the other hand, if you’re suddenly given coffee at a time when you’re not expecting it, then your body hasn’t got ready to receive this dose of caffeine and it has a much bigger effect than you Expect – and so it maybe not so much that people are addicted to coffee, but they are counteracting the negative effects of what’s in their body.

Caffeine is very soluble in hot water, there’s much much less than 30 times less soluble in room-temperature water. So we’re going to cool it down, so it’s less soluble in water, hydroxide, solution, drinking tea and coffee is very important in science, in the UK and in many labs people meet together every day to drink tea and coffee, and some of the best ideas can grow Up over tea and coffee because when people are relaxed, suddenly clever ideas come in their head.

An unexpected side effect of caffeine is its role in science, not directly, but in many labs. Many departments like this one at Nottingham. The scientists meet together once or perhaps twice a day to drink coffee, and quite often they have really good ideas, the caffeine of relaxes them and when they’re, not thinking really hard. Suddenly a good idea comes into their head. In fact, at the laboratory of molecular biology in Cambridge, which has won, I think, 14 Nobel prizes – there was a rule. Perhaps there still is that if people are discussing in the coffee room, it’s not allowed to close in case they’re just about to get a good idea and if the coffee room closes, it may be lost forever.

So you never know if you sit a long time drinking coffee, you might win the Nobel Prize, the water water’s more dense than ether estate, so it sinks to the bottom: they’re, not miscible, so we’re just draining off that bottom layer. At the moment, the water layer, [ Music, ] and, as it goes down you’ve seen the top layer is actually fairly clear. So the caffeine now hopefully, is in that top layer. Flask, there’s still some water in the philosophy, so we’re going to use is a drying agent, magnesium, sulfate, otherwise known as epsom salts. It’S a student’s often asked me. How do you know how much magnesium sulfate ready to dry?

And the answer is when it goes completely white and fluffy, so it’s quite long water and they see its client using clump together. So it’s put a bit more in says when you get that sort of snow storm effect, just let that dry for a moment filter off. So what we have here is our caffeine, so the Reds here deny oxygen. You’Ve got carbon atoms represented by the black. These smaller white ones are hydrogen and then the blue ones here which you’ve got for our nitrogen.

So it’s kind of the most common elements that make up so many aromatics or organic molecules. I tried to find a mug with a caffeine molecule in here to go back to the parent table, but what we had sent in by one of our viewers, this gentleman here is clearly a fan of periodic videos and he’s there enjoying a video of Martin with His broom in hand, but he also included in his email to us tattoo, that he’s got as you can see there, the molecule of caffeine. So it’s clearly a much more devoted fan to the molecule than I am. I just prefer drinking it, or rather than inking it on myself. Okay. So now our caffeine is in the flat state solution and we’re now going to remove these last eight from it. So you put one of these on. These are called Keck clips and dr

Keck, sadly, died last year, but very handy clip that bears his name will go on for many many years to come, but no need to car dice.

Okay, so this carebye approach to how to do it. So here we’ve got some solid sake, so we’re gon na use that condense, the ether state, so it doesn’t go down the drains or into the atmosphere. Okay. So what we’re doing is we’re lowering the pressure in the system. So that means that allowing the boiling point we’re lowering the boiling point of the organic solvent methyl acetate.

So in here we’ve got youthful acetate very low pressure in here currently around 90 milli bars, and that’s all that this evaporating that’s coming up here and it’s condensing on this very cold card ice cold finger here and you can see it coming down and being collected In the flask below so what we want to do is to remove all of that solvent and just leave what was insulted. I hope we’ll have some in there. I don’t hear it very much because it was only six cups of coffee, but there should be 20 or 30 milligrams, maybe a little bit more for lucky. So what do you think about someone?

I’Ve done one in himself with a caffeine molecule. It’S certainly a level of geekdom that I have not subscribed to, but I commend him for his devotion to this particular molecule. Well, Sam. That’S about to change, because we’ve been sent, something which I’m giving to you as a gift. This was sent to periodic videos mainly well. This is absolutely incredible. Oh, my gosh, so where you’ve got rid of most of the ether acetate now just the last few dregs coming off and you can see that we’ve got a fairly light, yellow page and some of the color has come through from the coffee. So there we go. Here’S some caffeine now if we were really good chemist, we’d recrystallized, that gets a nice white crystals and what we’re going to do is just going to put this on a high vacuum system to get the very last traces of ethyl acetate.

This is absolutely gorgeous. I’Ve got a caffeine molecule on a chain, it’s a necklace evidently, but it’s so cool. What is this? Oh? No! It’S made of. I absolutely adore this. It is it’s incredible and it’s so Daris, it’s a dose caffeine, but it’s so sweet. It’S so cute, and I love it, but you just you just said people who have done themselves with care yeah. I know I take it right now that I look like a complete idiot at all right, there’s a difference between getting inked permanently and being able to take it on and off. I’M gon na wear this because I I’m going to the Webby Awards and I’m gon na wear this. I’M definitely gon na wear this on the night. It’S going to be amazing. I had planned on wearing something else, but now I’m definitely going to take this to New York and I’m going to wear it for the Webby Awards, because it’s just it’s absolutely immense. I love it.

Thank you to whoever made this it’s now. It’S been on there for five minutes, so we’ll take this off now: okay, wait! Oh 100 milligrams, so six cups of coffee has 100 milligrams of caffeine, 17, also milligrams in a cup of that particular coffee. So in its amorphous powder form, [, Music, ],

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